Dental Materials
Volume 26, Issue 10 , Pages 1017-1023 , October 2010

Cytotoxicity of monomers, plasticizer and degradation by-products released from dental hard chairside reline resins

  • Carolina de Andrade Lima Chaves

      Affiliations

    • UNESP – Univ Estadual Paulista, Araraquara Dental School, Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680, Araraquara, CEP 14801-903, São Paulo, Brazil
    • ,
  • Ana Lucia Machado

      Affiliations

    • UNESP – Univ Estadual Paulista, Araraquara Dental School, Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680, Araraquara, CEP 14801-903, São Paulo, Brazil
    • Corresponding Author InformationCorresponding author at: UNESP – Univ Estadual Paulista, Araraquara Dental School, Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680, Araraquara, CEP 14801-903, São Paulo, Brazil. Tel.: +55 16 3301 6410; fax: +55 16 3301 6406.
    • ,
  • Iracilda Zeppone Carlos

      Affiliations

    • UNESP – Univ Estadual Paulista, Araraquara Pharmaceutical School, Department of Clinical Analysis, Araraquara, São Paulo, Brazil
    • ,
  • Eunice Teresinha Giampaolo

      Affiliations

    • UNESP – Univ Estadual Paulista, Araraquara Dental School, Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680, Araraquara, CEP 14801-903, São Paulo, Brazil
    • ,
  • Ana Cláudia Pavarina

      Affiliations

    • UNESP – Univ Estadual Paulista, Araraquara Dental School, Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680, Araraquara, CEP 14801-903, São Paulo, Brazil
    • ,
  • Carlos Eduardo Vergani

      Affiliations

    • UNESP – Univ Estadual Paulista, Araraquara Dental School, Department of Dental Materials and Prosthodontics, Rua Humaitá, 1680, Araraquara, CEP 14801-903, São Paulo, Brazil

Received 18 May 2010 ,Accepted 25 June 2010.

References 

  1. Dahl JE, Frangou-Polyzois MJ, Polyzois GL. In vitro biocompatibility of denture relining materials. Gerodontology. 2006;23:17–22
  2. Sofou A, Tsoupi I, Emmanouil J, Karayannis M. HPLC determination of residual monomers released from heat-cured acrylic resins. Anal Bioanal Chem. 2005;381:1336–1346
  3. Kedjarune U, Charoenworaluk N, Koontongkaew S. Release of methyl methacrylate from heat-cured and autopolymerized resins: cytotoxicity testing related to residual monomer. Aust Dent J. 1999;44:25–30
  4. Urban VM, Machado AL, Oliveira RV, Vergani CE, Pavarina AC, Cass QB. Residual monomer of reline acrylic resins. Effect of water-bath and microwave post-polymerization treatments. Dent Mater. 2007;23:363–368
  5. Lygre F, Solheim E, Gjerdet NR. Leaching from denture base materials in vitro. Acta Odontol Scand. 1995;53:75–80
  6. Lefebvre CA, Schuster GS. Biocompatibility of visible light-cured resin systems in prosthodontics. J Prosthet Dent. 1994;71:178–185
  7. Lai YL, Chen YT, Lee SY, Shieh TM, Hung SL. Cytotoxic effects of dental resin liquids on primary gingival fibroblasts and periodontal ligament cells in vitro. J Oral Rehabil. 2004;31:1165–1172
  8. Campanha NH, Pavarina AC, Giampaolo ET, Machado AL, Carlos IZ, Vergani CE. Cytotoxicity of hard chairside reline resins: effect of microwave irradiation and water bath postpolymerization treatments. Int J Prosthodont. 2006;19:195–201
  9. Jorge JH, Giampaolo ET, Vergani CE, Machado AL, Pavarina AC, Carlos IZ. Biocompatibility of denture base acrylic resins evaluated in culture of L929 cells. Effect of polymerisation cycle and post-polymerisation treatments. Gerodontology. 2007;24:52–57
  10. Weaver R, Goebel WM. Reactions to acrylic resin dental prostheses. J Prosthet Dent. 1980;43:138–142
  11. Ali A, Bates JF, Reynolds AJ, Walker DM. The burning mouth sensation related to the wearing of acrylic dentures: an investigation. Br Dent J. 1986;161:444–447
  12. Lunder T, Rogl-Butina M. Chronic urticaria from an acrylic dental prosthesis. Contact Dermatitis. 2000;43:232–233
  13. Bohnenkamp DM. Traumatic stomatitis following an intraoral denture reline: a clinical report. J Prosthet Dent. 1996;72:113–114
  14. Barron DJ, Schuster GS, Caughman GB, Lefebvre CA. Biocompatibility of visible light-polymerized denture base resins. Int J Prosthodont. 1993;6:495–501
  15. Hashimoto Y, Moriguchi Y, Oshima H, Nishikawa Y, Nishihara T, Nakamura M. Estrogenic activity of chemicals for dental and similar use in vitro. J Mater Sci. 2000;11:465–468
  16. Jobling S, Reynolds T, White R, Parker MG, Sumpter JP. A variety of environmentally persistent chemicals, including some phthalate plasticizers, are weakly estrogenic. Environ Health Perspect. 1995;103:582–587
  17. Kim SH, Kim SS, Kwon O, Sohn KH, Kwack SJ, Choi YO, et al. Effect of dibutyl phthalate and monobutyl phthalate on cytotoxicity and differentiation in cultured rat embryonic limb bud cells; protection by antioxidants. J Toxicol Environ Health A. 2002;65:461–472
  18. Xiang-Rong X, Huan-Bin L, Ji-Dong G, Xiao-Yan L. Kinetics of n-butyl benzyl phthalate degradation by a pure bacterial culture from the mangrove sediment. J Hazard Mater. 2007;140:194–199
  19. Seiss M, Nitz S, Kleinsasser N, Buters JTM, Behrendt H, Hickel R, et al. Identification of 2,3-epoxymethacrylic acid as an intermediate in the metabolism of dental materials in human liver microsomes. Dent Mater. 2007;23:9–16
  20. Emmler J, Seiss M, Kreppel H, Reichl FX, Hickel R, Kehe K. Cytotoxicity of dental composite TEGMA and selected metabolic by-products in human pulmonary cells. Dent Mater. 2008;24:1670–1675
  21. Koda T, Tsuchiya H, Yamauchi M, Ohtani S, Takagi N, Kawano J. Leachability of denture base acrylic resins in artificial saliva. Dent Mater. 1990;6:13–16
  22. Babich H, Zuckerbraun HL, Wurzburguer BJ, Rubin YL, Borenfreund E, Blau L. Benzoyl peroxide cytotoxicity evaluated in vitro with the human keratinocyte cell line RHEK-1. Toxicology. 1996;106:187–196
  23. Datar R, Fredrick AR, Caughman GB, Wataha JC, Lewis J, Schuster GS. Effects of subtoxic concentrations of benzoyl peroxide on cell lipid metabolism. J Biomed Mater Res. 2004;71:685–692
  24. Urban VM. Efeito de tratamento pós-polimerização sobre o peso molecular, grau de conversão, temperatura de transição vítrea e liberação in vitro de monômero residual, plastificante e produtos de degradação de resinas acrílicas para reembasamento imediato [Tese doutorado]. Araraquara: Faculdade de Odontologia da UNESP; 2007;
  25. Urban VM, Machado AL, Vergani CE, Giampaolo ET, Pavarina AC, Almeida FG, et al. Effect of water-bath post-polymerization on the mechanical properties, degree of conversion, and leaching of residual compounds of hard chairside reline resins. Dent Mater. 2009;25:662–671
  26. Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater. 2002;18:318–323
  27. Wataha JC, Hanks CT, Sun Z. Effect of cell line on in vitro metal ion cytotoxicity. Dent Mater. 1994;10:156–161
  28. International Standards Organization . ISO 10993-5: biological evaluation of medical devices—part 5. Geneva: ISO; 1999;
  29. Tang ATH, Li J, Eksrand J, Liu Y. Cytotoxicity tests of in situ polymerized resins: methodological comparisons and introduction of a tissue culture insert as a testing device. J Biomed Mater Res. 1999;45:214–222
  30. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Method. 1983;65:55–63
  31. Wataha JC, Hanks CT, Craig RG. The in vitro effects of metal cations on eukaryotic cell metabolism. J Biomed Mater Res. 1991;25:1133–1149
  32. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mater Res. 1997;37:517–524
  33. Imazato S, Horikawa D, Nishida M, Ebisu S. Effects of monomers eluted from dental resin restoratives on osteoblast-like cells. J Biomed Mater Res B: Appl Biomater. 2009;88:378–386
  34. Sun ZL, Wataha JC, Hanks CT. Effects of metal ions on osteoblast-like cell metabolism and differentiation. J Biomed Mater Res. 1997;34:29–37
  35. Meier B. Reactive oxygen intermediates involved in cellular regulation. Protoplasma. 2001;217:101–116
  36. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res. 1998;41:474–480
  37. Wataha JC, Craig RG, Hanks CT. Precision of and new methods for testing in vitro alloy cytotoxicity. Dent Mater. 1992;8:65–70
  38. Fujisawa S, Kadoma Y, Komoda Y. 1H and 13C NMR studies of the interaction of eugenol, phenol, and triethyleneglycol dimethacrylate with phospholipid liposomes as a model system for odontoblast membranes. J Dent Res. 1988;67:1438–1441
  39. Silikas N, Watts DC. High pressure liquid chromatography of dentin primers and bonding agents. Dent Mater. 2000;16:81–88
  40. Atsumi T, Fujisawa S, Tonosaki K. (Meth)acrylate monomer-induced cytotoxicity and intracellular Ca(2+) mobilization in human salivary gland carcinoma cells and human gingival fibroblast cells related to monomer hydrophobicity. Biomaterials. 2006;27:5794–5800
  41. Urban VM, Cass QB, Oliveira RV, Giampaolo ET, Machado AL. Development and application of methods for determination of residual monomer in dental acrylic resins using high performance liquid chromatography. Biomed Chromatogr. 2006;20:369–376
  42. Mainwaring G, Foster JR, Lud V, Green T. Methyl methacrylate toxicity in rat nasal epithelium: studies of the mechanism of action and comparisons between species. Toxicology. 2001;158:109–118
  43. Schwengberg S, Bohlen H, Kleinsasser N, Kehe K, Seiss M, Walther UI, et al. In vitro embryotoxicity assessment with dental restorative materials. J Dent. 2005;33:49–55
  44. De Groot CJ, Van Luyn MJ, Van Dijk-Wolthuis WN, Cadée JA, Plantinga JA, Den Otter W, et al. In vitro biocompatibility of biodegradable dextran-based hydrogels tested with human fibroblasts. Biomaterials. 2001;22:1197–1203
  45. Henriques M, Quintas C, Loureiro-Dias MC. Extrusion of benzoic acid in Saccharomyces cerevisiae by an energy-dependent mechanism. Microbiology. 1997;143:1877–1883
  46. Swauger JE, Dolan PM, Zweier JL, Kuppusamy P, Kensler TW. Role of the benzoyloxyl radical in DNA damage mediated by benzoyl peroxide. Chem Res Toxicol. 1991;4:223–228
  47. Milan KM, Cleaver JE. Inhibitors of poly(adenosine diphosphate–ribose) synthesis: effect on other metabolic processes. Science. 1983;223:589–591

PII: S0109-5641(10)00169-7

doi: 10.1016/j.dental.2010.06.008

Dental Materials
Volume 26, Issue 10 , Pages 1017-1023 , October 2010

Article Tools

* Image Usage & Resolution