Dental Materials
Volume 26, Issue 4 , Pages 320-325, April 2010

Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study

  • Lorenzo Breschi

      Affiliations

    • Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Italy
    • IGM-CNR, Unit of Bologna c/o IOR, Bologna, Italy
    • Corresponding Author InformationCorresponding author at: Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Via Stuparich 1, I-34129 Trieste, Italy. Tel.: +39 040 3992192; fax: +39 040 3992665.
  • ,
  • Annalisa Mazzoni

      Affiliations

    • Department of SAU&FAL, University of Bologna, Bologna, Italy
  • ,
  • Fernando Nato

      Affiliations

    • Department of SAU&FAL, University of Bologna, Bologna, Italy
    • Department of SUAN, University “Carlo Bo”, Urbino, Italy
  • ,
  • Marcela Carrilho

      Affiliations

    • Department of Restorative Dentistry, Piracicaba School of Dentistry, University of Campinas, Piracicaba, Brazil
    • Bandeirante University of São Paulo (UNIBAN), São Paulo, Brazil
  • ,
  • Erika Visintini

      Affiliations

    • Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Italy
  • ,
  • Leo Tjäderhane

      Affiliations

    • Institute of Dentistry, University of Oulu and Oulu University Hospital (OUH), Oulu, Finland
  • ,
  • Alessandra Ruggeri Jr

      Affiliations

    • Department of SAU&FAL, University of Bologna, Bologna, Italy
  • ,
  • Franklin R. Tay

      Affiliations

    • Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA, USA
  • ,
  • Elettra De Stefano Dorigo

      Affiliations

    • Department of Biomedicine, Unit of Dental Sciences and Biomaterials, University of Trieste, Trieste, Italy
  • ,
  • David H. Pashley

      Affiliations

    • Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA, USA

Received 2 July 2009; accepted 24 November 2009.

Abstract 

Objectives

This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time.

Methods

Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2–2% CHX-treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30s on the etched surface) were analyzed immediately and after 2 years of storage in artificial saliva at 37°C.

Results

Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 years.

Significance

The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2%).

Keywords: Chlorhexidine, Dental bonding systems, Hybrid layer, Aging, Dentin

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PII: S0109-5641(09)00499-0

doi:10.1016/j.dental.2009.11.153

Dental Materials
Volume 26, Issue 4 , Pages 320-325, April 2010