Dental Materials
Volume 24, Issue 9 , Pages 1212-1222 , September 2008

Injectable and strong nano-apatite scaffolds for cell/growth factor delivery and bone regeneration

  • Hockin H.K. Xu

      Affiliations

    • Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201-1586, USA
    • Corresponding Author InformationCorresponding author at: Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, 650 West Baltimore Street, Baltimore, MD 21201, USA. Tel.: +1 410 706 7047; fax: +1 410 706 3028.
    • ,
  • Michael D. Weir

      Affiliations

    • Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201-1586, USA
    • ,
  • Carl G. Simon

      Affiliations

    • National Institute of Standards and Technology, Gaithersburg, MD 20899-8546, USA

Received 16 January 2008 ,Accepted 2 February 2008.

References 

  1. Praemer A, Furner S, Rice DP. Musculoskeletal conditions in the United States. Rosemont: Amer Acad Orthop Surg; 1999 [chapter 1].
  2. Franceshi RT. Biological approaches to bone regeneration by gene therapy. Crit Rev Oral Biol Med. 2005;84:1093–1103
  3. Medical Data International. RP-651147—Orthopedic and musculoskeletal markets: Biotechnology and Tissue Engineering. 2000; p. 28–31.
  4. Ambrosio AMA, Sahota JS, Khan Y, Laurencin CT. A novel amorphous calcium phosphate polymer ceramic for bone repair. I. Synthesis and characterization. J Biomed Mater Res. 2001;58B:295–301
  5. Laurencin CT, Ambrosio AMA, Borden MD, Cooper JA. Tissue engineering: orthopedic applications. Annu Rev Biomed Eng. 1999;1:19–46
  6. Hench LL, Polak JM. Third-generation biomedical materials. Science. 2002;295:1014–1017
  7. An YH. Internal fixation in osteoporotic bone. New York, NY: Thieme Medical, 2002, Foreword.
  8. Murphy WL, Hsiong S, Richardson TP, Simmons GA, Mooney DJ. Effects of a bone-like mineral film on phenotype of adult human mesenchymal stem cells in vitro. Biomaterials. 2005;26:303–310
  9. Livingston T, Ducheyne P, Garino J. In vivo evaluation of a bioactive scaffold for bone tissue engineering. J Biomed Mater Res. 2002;62:1–13
  10. Ehara A, Ogata K, Imazato S, Ebisu S, Nakano T, Umakoshi Y. Effects of alpha-TCP and TetCP on MC3T3-E1 proliferation, differentiation and mineralization. Biomaterials. 2003;24:831–836
  11. LeGeros RZ. Biodegradation and bioresorption of calcium phosphate ceramics. Clin Mater. 1993;14:65–88
  12. Suchanek W, Yoshimura M. Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J Mater Res. 1998;13:94–117
  13. Hing KA, Best SM, Bonfield W. Characterization of porous hydroxyapatite. J Mater Sci: Mater Med. 1999;10:135–145
  14. Ducheyne P, Qiu Q. Bioactive ceramics: the effect of surface reactivity on bone formation and bone cell function. Biomaterials. 1999;20:2287–2303
  15. Pilliar RM, Filiaggi MJ, Wells JD, Grynpas MD, Kandel RA. Porous calcium polyphosphate scaffolds for bone substitute applications – in vitro characterization. Biomaterials. 2001;22:963–972
  16. Chu TMG, Orton DG, Hollister SJ, Feinberg SE, Halloran JW. Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures. Biomaterials. 2002;23:1283–1293
  17. Foppiano S, Marshall SJ, Marshall GW, Saiz E, Tomsia AP. The influence of novel bioactive glasses on in vitro osteoblast behavior. J Biomed Mater Res A. 2004;71:242–249
  18. Russias J, Saiz E, Deville S, Gryn K, Liu G, Nalla RK, et al. Fabrication and in vitro characterization of three-dimensional organic/inorganic scaffolds by robocasting. J Biomed Mater Res A. 2007;83:434–445
  19. Brown WE, Chow LC. A new calcium phosphate water setting cement. In:  Brown PW editors. Cements research progress. Westerville, OH: American Ceramic Society; 1986;p. 352–379
  20. Ginebra MP, Fernandez E, De Maeyer EAP, Verbeeck RMH, Boltong MG, Ginebra J, et al. Setting reaction and hardening of an apatite calcium phosphate cement. J Dent Res. 1997;76:905–912
  21. Ishikawa K, Miyamoto Y, Takechi M, Toh T, Kon M, Nagayama M, et al. Non-decay type fast-setting calcium phosphate cement: hydroxyapatite putty containing an increased amount of sodium alginate. J Biomed Mater Res. 1997;36:393–399
  22. Durucan C, Brown PW. Low temperature formation of calcium-deficient hydroxyapatite-PLA/PLGA composites. J Biomed Mater Res. 2000;51:717–725
  23. Barralet JE, Gaunt T, Wright AJ, Gibson IR, Knowles JC. Effect of porosity reduction by compaction on compressive strength and microstructure of calcium phosphate cement. J Biomed Mater Res B. 2002;(63):1–9
  24. Shindo ML, Costantino PD, Friedman CD, Chow LC. Facial skeletal augmentation using hydroxyapatite cement. Arch Otolaryngol Head Neck Surg. 1993;119:185–190
  25. Friedman CD, Costantino PD, Takagi S, Chow LC. BoneSource hydroxyapatite cement: a novel biomaterial for craniofacial skeletal tissue engineering and reconstruction. J Biomed Mater Res (Appl Biomater). 1998;43:428–432
  26. Chow LC. Calcium phosphate cements: chemistry, properties, and applications. Mater Res Symp Proc. 2000;599:27–37
  27. Brown GD, Mealey BL, Nummikoski PV, Bifano SL, Waldrop TC. Hydroxyapatite cement implant for regeneration of periodontal osseous defects in humans. J Periodontol. 1998;69:146–157
  28. Shors EC, Holmes RE. Porous hydroxyapatite. In:  Hench LL,  Wilson J editor. An introduction to bioceramics. New Jersey: World Scientific; 1993;p. 181–198
  29. Xu HHK, Quinn JB. Calcium phosphate cement containing resorbable fibers for short-term reinforcement and macroporosity. Biomaterials. 2002;23:193–202
  30. Xu HHK, Quinn JB, Takagi S, Chow LC, Eichmiller FC. Strong and macroporous calcium phosphate cement: effects of porosity and fiber reinforcement on mechanical properties. J Biomed Mater Res. 2001;57:457–466
  31. Takagi S, Chow LC. Formation of macropores in calcium phosphate cement implants. J Mater Sci: Mater Med. 2001;12:135–139
  32. Xu HHK, Simon CG. Self-hardening calcium phosphate composite scaffold for bone tissue engineering. J Orthop Res. 2004;22:535–543
  33. Burguera EF, Xu HHK, Takagi S, Chow LC. High early-strength calcium phosphate bone cement: effects of dicalcium phosphate dehydrate and absorbable fibers. J Biomed Mater Res A. 2005;75:966–975
  34. Burguera EF, Xu HHK, Weir MD. Injectable and rapid-setting calcium phosphate bone cement with dicalcium phosphate dihydrate. J Biomed Mater Res B. 2006;77:126–134
  35. Xu HHK, Eichmiller FC, Giuseppetti AA. Reinforcement of a self-setting calcium phosphate cement with different fibers. J Biomed Mater Res. 2000;52:107–114
  36. Xu HHK, Braum LM, Ostertag CP, Krause RF, Lloyd IK. Failure mode of SiC-fiber-reinforced Si3N4 composites at elevated temperatures. J Am Ceram Soc. 1995;78:388–394
  37. Xu HHK, Ostertag CP, Braun LM, Lloyd IK. Effects of fiber volume fraction on mechanical properties of SiC-fiber/Si3N4-matrix composites. J Am Ceram Soc. 1994;77:1897–1900
  38. Zhang Y, Xu HHK. Effects of synergistic reinforcement and absorbable fiber strength on hydroxyapatite bone cement. J Biomed Mater Res. 2005;75A:832–840
  39. Zhang Y, Xu HHK, Takagi S, Chow LC. In-situ hardening hydroxyapatite-based scaffold for bone repair. J Mater Sci: Mater Med. 2006;17:437–445
  40. International Standards Organization. ISO 10993-5. Biological evaluation of medical devices—Part 5: Tests for in vitro cytotoxicity. International Standards Organization, Geneva, Switzerland, 1999.
  41. Simon CG, Khatri CA, Wight SA, Wang FW. Preliminary report on the biocompatibility of a moldable, resorbable, composite bone graft consisting of calcium phosphate cement and poly(lactide-co-glycolide) microspheres. J Orthop Res. 2002;20:473–482
  42. Xu HHK, Smith DT, Simon CG. Strong and bioactive composites containing nano-silica-fused whiskers for bone repair. Biomaterials. 2004;25:4615–4626
  43. Xu HHK, Simon CG. Fast setting calcium phosphate-chitosan scaffold: mechanical properties and biocompatibility. Biomaterials. 2005;26:1337–1348
  44. Boontheekul T, Kong HJ, Mooney DJ. Controlling alginate gel degradation utilizing partial oxidation and bimodal molecular weight distribution. Biomaterials. 2005;26:2455–2465
  45. Weir MD, Xu HHK, Simon CG. Strong calcium phosphate cement-chitosan-mesh construct containing cell-encapsulating hydrogel beads for bone tissue engineering. J Biomed Mater Res A. 2006;77:487–496
  46. Lee DD, Tofighi A, Aiolova M, Chakravarthy P, Catalano A, Majahad A, et al. α-BSM: A biomimetic bone substitute and drug delivery vehicle. Clin Orthop Res. 1999;367S:S396–S405
  47. Blom EJ, Klein-Nulend J, Wolke JGC, Kurashina K, van Waas MAJ, Burger EH. Transforming growth factor-β1 incorporation in an β-tricalcium phosphate/dicalcium phosphate dehydrate/tetracalcium phosphate monoxide cement: release characteristics and physiochemical properties. Biomaterials. 2002;23:1261–1268
  48. Ruhe PQ, Hedberg EL, Padron NT, Spauwen PHM, Jansen JA, Mikos AG. rhBMP-2 release from injectable poly(dl-lactic-co-glycolic acid)/calcium-phosphate cement composites. J Bone Joint Surg. 2003;85:75–81
  49. Huang YC, Kaigler D, Rice KG, Krebsbach PH, Mooney DJ. Combined angiogenic and osteogenic factor delivery enhances bone marrow stromal cell-driven bone regeneration. J Bone Miner Res. 2005;20:848–857
  50. Isobe M, Yamazaki Y, Mori M, Ishihara K, Nakabayashi N, Amagasa T. The role of recombinant human bone morphogenetic protein-2 in PLGA capsules at an extraskeletal site of the rat. J Biomed Mater Res. 1999;45:36–41
  51. Paralkar VM, Hammonds RG, Reddi AH. Identification and characterization of cellular binding proteins (receptors) for recombinant human bone morphogenetic protein 2B, an initiator of bone differentiation cascade. Proc Natl Acad Sci USA. 1991;15(88):3397–3401
  52. R&D Systems. www.RnDSystems.com. Recombinant human BMP-2: Specifications and use. Catalog number: 355-BM, 2005.
  53. Weir MD, Xu HHK. High-strength, in situ-setting calcium phosphate composite with protein release. J Biomed Mater Res, in press.
  54. Seeherman H, Wozney JM. Delivery of bone morphogenetic proteins for orthopedic tissue regeneration. Cytokine Growth Factor Rev. 2005;16:329–345
  55. Cunningham NS, Paralkar V, Reddi AH. Osteogenin and recombinant bone morphogenetic protein 2B are chemotactic for human monocytes and stimulate transforming growth factor beta 1 mRNA expression. Proc Natl Acad Sci USA. 1992;89:11740–11744
  56. Kim HD, Wozney JM, Li RH. Characterization of a calcium phosphate-based matrix for rhBMP-2. Methods Mol Biol. 2004;238:49–64

☆ This article was presented as an invited talk at the Academy of Dental Materials Annual Meeting in Fort Lauderdale, FL, USA, October 2007.

☆☆ Certain commercial materials and equipment are identified in this paper to specify experimental procedures. In no instance does such identification imply recommendation by NIST. One standard deviation (S.D.) was used as the estimated standard uncertainty of the measurements unless otherwise specified in the Methods. These data should not be compared with those obtained in other laboratories under different conditions. Official contribution of the National Institute of Standards and Technology; not subject to copyright in the United States.

PII: S0109-5641(08)00033-X

doi: 10.1016/j.dental.2008.02.001

Dental Materials
Volume 24, Issue 9 , Pages 1212-1222 , September 2008

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