Dental Materials
Volume 24, Issue 9 , Pages 1201-1206, September 2008

Cytotoxicity of silica–glass fiber reinforced composites

  • Gökçe Meriç

      Affiliations

    • Department of Prosthetic Dentistry, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway
    • NIOM, Nordic Institute of Dental Materials, Haslum, Norway
    • ,
  • Jon E. Dahl

      Affiliations

    • NIOM, Nordic Institute of Dental Materials, Haslum, Norway
    • ,
  • I. Eystein Ruyter

      Affiliations

    • NIOM, Nordic Institute of Dental Materials, Haslum, Norway
    • Corresponding Author InformationCorresponding author at: NIOM, Nordic Institute of Dental Materials, P.O. Box 70, NO-1305, Haslum, Norway. Tel.: +47 67 51 22 09; fax: +47 67 59 15 30.

Received 25 June 2007; received in revised form 23 January 2008; accepted 29 January 2008.

Abstract 

Objectives

Silica–glass fiber reinforced polymers can be used for many kinds of dental applications. The fiber reinforcement enhances the mechanical properties of the polymers, and they have good esthetic attributes. There is good initial bonding of glass fibers to polymers via an interface made from silane coupling agents. The aim of this in vitro study was to determine the cytotoxicity of two polymers reinforced with two differently sized silica–glass fibers before and after thermal cycling. Cytotoxicity of the polymers without fibers was also evaluated.

Methods

Two different resin mixtures (A and B) were prepared from poly(vinyl chloridecovinylacetate) powder and poly(methyl methacrylate) (PMMA) dissolved in methyl methacrylate and mixed with different cross-linking agents. The resin A contained the cross-linking agents ethylene glycol dimethacrylate and 1,4-butanediol dimethacrylate, and for resin B diethylene glycol dimethacrylate was used. Woven silica–glass fibers were used for reinforcement. The fibers were sized with either linear poly(butyl methacrylate)-sizing or cross-linking PMMA-sizing. Cytotoxicity was evaluated by filter diffusion test (ISO 7405:1997) of newly made and thermocycled test specimens. Extracts were prepared according to ISO 10993-12 from newly made and from thermocycled specimens and tested by the MTT assay. The results from the experiments were statistically analyzed by one-way ANOVA and Tukey's test (ρ<0.05).

Results

The filter diffusion test disclosed no change in staining intensity at the cell-test sample contact area indicating non-cytotoxicity in all experimental groups. Cell viability assessed by MTT assay was more than 90% in all experimental groups. All are non-cytotoxic.

Significance

It can be concluded that correctly processed heat polymerized silica–glass fiber reinforced polymers induced no cytotoxicity and that thermocycling did not alter this property.

Keywords: Biocompatibility, Polymer, Residual monomer, Silanization, Sizing

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PII: S0109-5641(08)00024-9

doi:10.1016/j.dental.2008.01.010

Dental Materials
Volume 24, Issue 9 , Pages 1201-1206, September 2008

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