Histomorphologic characterization and bond strength evaluation of caries-affected dentin/resin interfaces: Effects of long-term water exposure

Erhardt, Maria Carolina G.; Toledano, Manuel; Osorio, Raquel; Pimenta, Luiz André

doi:10.1016/j.dental.2007.09.007

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Volume 24, Issue 6 , Pages 786-798, June 2008

Histomorphologic characterization and bond strength evaluation of caries-affected dentin/resin interfaces: Effects of long-term water exposure

Maria Carolina G. Erhardt
- Department of Restorative Dentistry, Division of Operative Dentistry, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, Brazil
- Department of Dental Materials, School of Dentistry, University of Granada, Granada, Spain
Manuel Toledano
- Department of Dental Materials, School of Dentistry, University of Granada, Granada, Spain
Raquel Osorio
- Department of Dental Materials, School of Dentistry, University of Granada, Granada, Spain
Luiz André Pimenta
- Department of Restorative Dentistry, Division of Operative Dentistry, School of Dentistry at Piracicaba, University of Campinas, Piracicaba, Brazil
- Department of Dental Ecology, School of Dentistry, University of North Carolina at Chapel Hill (UNC), USA
- Corresponding author at: School of Dentistry UNC at Chapel Hill, Department of Dental Ecology – DOB 316, Manning Dr. & Columbia St., Chapel Hill, NC, USA. Tel.: +1 919 9662787; fax: +1 919 966 6761.

Received 8 May 2007; received in revised form 10 September 2007; accepted 29 September 2007.

Abstract

Objectives

To evaluate the longevity of sound (SD) and caries-affected dentin (CAD) bonds made with etch-and-rinse and self-etching adhesives after a 6-month water-storage period, using bond strength and morphological evaluations.

Methods

Extracted human molars with coronal carious lesions were selected. Flat surfaces of CAD surrounded by SD were bonded with etch-and-rinse (Adper Scotchbond 1) or with self-etching (Clearfil Protect Bond and AdheSE) adhesives. Trimmed resin–dentin bonded interfaces (1mm²) were stored in distilled water for 24h or 6 months and subjected to microtensile bond strength (μTBS) evaluation. The quality of the dentin beneath fractured specimens was measured by Knoop microhardness (KHN). ANOVA and multiple comparisons tests were used (P<0.05). Fractographic analysis and interfacial nanoleakage evaluation were performed by scanning electron microscopy (SEM). Resin–dentin bonded sections (10μm thick) were stained with Masson's trichrome and examined using light microscopy. Collagen exposure and adhesive penetration were examined qualitatively.

Results

μTBS to SD was significantly higher than that to CAD for all bonding agents. Bonds made with AdheSE were weaker than the other adhesives after 6-months storage regardless of the dentin substrate. CAD bonded specimens presented a significant μTBS decrease over time. Lower KHN was recorded in CAD compared to SD. An increase in the exposed collagen zone and a decrease in the quality of the adhesive infiltration were observed in CAD interfaces.

Significance

CAD bonded interfaces are more prone to hydrolytic degradation than SD bonds. Additionally, as compared to SD, there were remarkable differences in depth of demineralization, adhesive infiltration and interfacial bond strength with CAD.

Keywords: Adhesion, Caries-affected dentin, Etch-and-rinse, Self-etching, Longevity, Microtensile bond strength, Hybrid layer, SEM, Collagen, Encapsulation