Dental Materials
Volume 23, Issue 1 , Pages 40-44 , January 2007

Cytotoxicity of resin monomers on human gingival fibroblasts and HaCaT keratinocytes

  • Keyvan Moharamzadeh

      Affiliations

    • School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK
    • Corresponding Author InformationCorresponding author. Tel.: +44 114 2717924; fax: +44 114 2665326.
  • ,
  • Richard Van Noort

      Affiliations

    • School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK
  • ,
  • Ian M. Brook

      Affiliations

    • School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK
  • ,
  • Andy M. Scutt

      Affiliations

    • Department of Engineering Materials, University of Sheffield, Mappin Street, Sheffield S1 3JD, UK

Received 14 July 2005 ,Accepted 30 November 2005.

References 

  1. Spahl W, Budzikiewicz H, Geurtsen W. Determination of leachable components from four commercial dental composites by gas and liquid chromatography/mass spectrometry. J Dent. 1998;26:137–145
  2. Hensten-Pettersen A. Skin and mucosal reactions associated with dental materials. Eur J Oral Sci. 1998;106:707–712
  3. Bouillaguet S, Shaw L, Gonzalez L, Wataha JC, Krejci I. Long-term cytotoxicity of resin-based dental restorative materials. J Oral Rehabil. 2002;29:7–13
  4. Willershausen B, Schafer D, Pistorius A, Schulze R, Mann W. Influence of resin-based restoration materials on cytotoxicity in gingival fibroblasts. Eur J Med Res. 1999;4:149–155
  5. Schmalz G. The biocompatibility of non-amalgam dental filling materials. Eur J Oral Sci. 1998;106:696–706
  6. Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla-derived cell lines to dental resin components. Dent Mater. 2002;18:318–323
  7. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res. 1998;41:474–480
  8. Nociari MM, Shalev A, Benias P, Russo C. A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity. J Immunol Methods. 1998;213:157–167
  9. Fields RD, Lancaster MV. Dual-attribute continuous monitoring of cell proliferation/cytotoxicity. Am Biotechnol Lab. 1993;11:48–50
  10. Jandinski JJ. Osteoclast activating factor is now interleukin-1 beta: historical perspective and biological implications. J Oral Pathol. 1988;17:145–152
  11. Noda M, Wataha JC, Lockwood PE, Volkmann KR, Kaga M, Sano H. Sublethal, 2-week exposures of dental material components alter TNF-alpha secretion of THP-1 monocytes. Dent Mater. 2003;19:101–105
  12. Schmalz G, Schweikl H, Hiller KA. Release of prostaglandin E2, IL-6 and IL-8 from human oral epithelial culture models after exposure to compounds of dental materials. Eur J Oral Sci. 2000;108:442–448
  13. Heil TL, Volkmann KR, Wataha JC, Lockwood PE. Human peripheral blood monocytes versus THP-1 monocytes for in vitro biocompatibility testing of dental material components. J Oral Rehabil. 2002;29:401–407
  14. Hensten-Pettersen A, Helgeland K. Sensitivity of different human cell line in the biologic evaluation of dental resin-based restorative materials. Scand J Dent Res. 1981;89:102–107
  15. Polyzois GL. In vitro evaluation of dental materials. Clin Mater. 1994;16:21–60
  16. Theilig C, Tegtmeier Y, Leyhausen G, Geurtsen W. Effects of BisGMA and TEGDMA on proliferation, migration, and tenascin expression of human fibroblasts and keratinocytes. J Biomed Mater Res. 2000;53:632–639
  17. Ferracane JL, Condon JR. Rate of elution of leachable components from composite. Dent Mater. 1990;6:282–287
  18. Reichl FX, Walther UI, Durner J, Kehe K, Hickel R, Kunzelmann KH, et al. Cytotoxicity of dental composite components and mercury compounds in lung cells. Dent Mater. 2001;17:95–101
  19. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602–1606
  20. Issa Y, Watts DC, Brunton PA, Waters CM, Duxbury AJ. Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro. Dent Mater. 2004;20:12–20
  21. Engelmann J, Leyhausen G, Leibfritz D, Geurtsen W. Effect of TEGDMA on the intracellular glutathione concentration of human gingival fibroblasts. J Biomed Mater Res. 2002;63:746–751
  22. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mater Res. 1997;37:517–524
  23. Geurtsen W, Spahl W, Leyhausen G. Residual monomer/additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers. J Dent Res. 1998;77:2012–2019
  24. Lefebvre CA, Schuster GS, Rueggeberg FA, Tamareselvy K, Knoernschild KL. Responses of oral epithelial cells to dental resin components. J Biomater Sci Polym Ed. 1996;7:965–976
  25. Spagnuolo G, Galler K, Schmalz G, Cosentino C, Rengo S, Schweikl H. Inhibition of phosphatidylinositol 3-kinase amplifies TEGDMA-induced apoptosis in primary human pulp cells. J Dent Res. 2004;83:703–707
  26. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A. TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res. 2003;66A:476–482
  27. Lefeuvre M, Bourd K, Loriot MA, Goldberg M, Beaune P, Perianin A, et al. TEGDMA modulates glutathione transferase P1 activity in gingival fibroblasts. J Dent Res. 2004;83:914–919
  28. Ortengren U, Wellendorf H, Karlsson S, Ruyter IE. Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment. J Oral Rehabil. 2001;28:1106–1115

PII: S0109-5641(05)00355-6

doi: 10.1016/j.dental.2005.11.039

Dental Materials
Volume 23, Issue 1 , Pages 40-44 , January 2007