Dental Materials
Volume 22, Issue 7 , Pages 630-640 , July 2006

Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts

  • Rune Becher

      Affiliations

    • Noraic Institute of Dental Materials, Haslum, Norway
    • Division of Environmental Medicine, Norwegian Institute of Public Health, Oslo, Norway
    • Corresponding Author InformationCorresponding author. Address: Division of Environmental Medicine, Norwegian Institute of Public Health, Geitmyrsveien 75, P.O. Box 4404 Nydalen, N-0403, Oslo, Norway. Tel.: +47 22 04 22 00; fax: +47 22 35 36 05.
  • ,
  • Hilde Molvig Kopperud

      Affiliations

    • Noraic Institute of Dental Materials, Haslum, Norway
    • Division of Environmental Medicine, Norwegian Institute of Public Health, Oslo, Norway
  • ,
  • Ronald H. Al

      Affiliations

    • Noraic Institute of Dental Materials, Haslum, Norway
    • Current address: Norwegian University of Life Sciences Ås, Norway.
  • ,
  • Jan Tore Samuelsen

      Affiliations

    • Noraic Institute of Dental Materials, Haslum, Norway
  • ,
  • Else Morisbak

      Affiliations

    • Noraic Institute of Dental Materials, Haslum, Norway
  • ,
  • Hans Jörgen Dahlman

      Affiliations

    • Division of Environmental Medicine, Norwegian Institute of Public Health, Oslo, Norway
  • ,
  • Edel Marie Lilleaas

      Affiliations

    • Division of Environmental Medicine, Norwegian Institute of Public Health, Oslo, Norway
  • ,
  • Jon E. Dahl

      Affiliations

    • Noraic Institute of Dental Materials, Haslum, Norway

Received 13 May 2004 ,Revised 11 May 2005 ,Accepted 24 May 2005.

References 

  1. Schuster GS, Lefebvre CA, Wataha JC, White SN. Biocompatibility of posterior restorative materials. J Calif Dent Assoc. 1996;24:17–31
  2. Schweikl H, Schmalz G. Toxicity parameters for cytotoxicity testing of dental materials in two different mammalian cell lines. Eur J Oral Sci. 1996;104:292–299
  3. Cohen GM. Caspases: the executioners of apoptosis. Biochem J. 1997;326:1–16
  4. Steller H. Mechanisms and genes of cellular suicide. Science. 1995;267:1445–1449
  5. Wyllie AH. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activity. Nature. 1980;284:555–556
  6. Ruse ND. What is a compomer?. J Can Dent Assoc. 1999;65:500–504
  7. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602–1606
  8. Yoshii E. Cytotoxic effects of acrylates and methacrylates: relationships of monomer structures and cytotoxicity. J Biomed Mater Res. 1997;37:517–524
  9. Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures. J Biomed Mater Res. 1998;41:474–480
  10. Sletten GBG, Dahl JE. Cytotoxic effects of extracts of compomers. Acta Odontol Scand. 1999;57:316–322
  11. Mathias CGT, Caldwell TM, Maibach HI. Contact dermatitis and gastrointestinal symptoms from hydroxyethylmethylmethacrylate. Br J Dermatol. 1987;100:447–449
  12. Lonnroth BC, Shahnavaz H. Use of polymer materials in dental clinics, case studies. Swed Dent J. 1997;21:149–159
  13. GoldberG NR, Goldberg AF, Gergans GA, Loga S, Taschini P, Molna ZV. A rabbit lung model for testing reaction to inhaled dental particles. Chest. 1992;101(3):829–832
  14. Caughman GB, Schuster GS, Rueggeberg FA. Cell lipid alterations resulting from prolonged exposure to dimethylaminoethylmethacrylate. Clin Oral Investig. 1999;3:181–187
  15. Geurtsen W, Spahl W, Leyhausen G. Residual monomer/additive release and variability in cytotoxicity of light-curing glass-ionomer cements and compomers. J Dent Res. 1998;77:2012–2019
  16. Hamid A, Okamoto A, Iwaku M, Hume WR. Component release from light-activated glass ionomer and compomer cements. J Oral Rehabil. 1998;25:94–99
  17. Michelsen VB, Lygre H, Skålevik R, Tveit AB, Solheim E. Identification of organic eluates from four polymer-based dental filling materials. Eur J Oral Sci. 2003;111(3):263–271
  18. Munksgaard EC, Freund M. Enzymatic hydrolysis of (Di) methacrylates and their polymers. Scand J Dent Res. 1990;98:261–267
  19. Bean TA, Zhuang WC, Tong PY, Eick JD, Yourtee DM. Effect of esterase on methacrylates and methacrylate polymers in an enzyme simulator for biodurability and biocompatibility testing. J Biomed Mater Res. 1994;28:59–63
  20. Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res. 1991;70:1450–1455
  21. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res. 1995;74:1602–1606
  22. Wataha JC, Hanks CT, Strawn SE, Fat JC. Cytotoxicity of components of resins and other dental resotrative materials. J Oral Rehabil. 1994;21:453–462
  23. Reichl F-X, Dumer J, Muckter H, Kunzelmann KH, Hickel R, Forth W. Effect of dental materials in rat kidney tubules. Arch Toxicol. 1999;73:381–386
  24. Spagnuolo G, Mauro C, Leonardi A, Santillo M, Paternò R, Schweikl H, et al. NF-B protection against apoptosis induced by HEMA. J Dent Res. 2004;83(11):837–842
  25. Spagnuolo G, Galler K, Schmalz G, Cosentino C, Rengo S, Schweikl H. Inhibition of phosphatidylinositol 3-Kinase amplifies TEGDMA-induced apoptosis in primary human pulp cells. J Dent Res. 2004;83(9):703–707
  26. Janke V, von Neuhoff N, Schlegelberger B, Leyhausen G, Geurtsen W. TEGDMA causes apoptosis in primary human gingival fibroblasts. J Dent Res. 2003;82(10):814–818
  27. Edmondson JM, Armstrong LS, Martinez AO. A rapid and simple MTT based sprctrophotometric assay for determining drug sensitivity in monolayer cultures. J Tissue Cult Methods. 1988;11:5–7
  28. Vindeløv LL, Christensen IJ, Nissen NI. Standardization of high resolution flow cytometric DNA analysis by the simultaneous use of chicken and trout red blood cells as internal reference standards. Cytometry. 1983;3:328–331
  29. Hse KMY, Leung SK, Wei SHY. Resin-ionomer restorative materials for children, a review. Aust Dent J. 1999;44(1):1–11
  30. Reichl FX, Walther UI, Kehe K, Kunzelmann KH, Spahl W, Hume WR, et al. Cytotoxicity of dental composite components and mercury compounds in lung cells. Dent Mater. 2001;17:95–101
  31. Cimpan RM, Cressey LI, Skaug N, Halstensen A, Lie SA, Gjertsen BT, et al. Patterns of cell death induced by eluates from denture base acrylic resins in U-937 human monoblastoid cells. Eur J Oral Sci. 2000;108:59–69
  32. Cotter TG, Martin SJ. Techniques in apoptosis—a users guide. 1st ed. London, UK: Portland Press Inc.; 1995;
  33. Thompson CB. Apoptosis in the pathogenesis and treatment of disease. Science. 1995;267:1456–1462
  34. Nagata S. Apoptosis by death factor. Cell. 1988;355–365
  35. Green DR. Apoptotic pathways: the roads to ruin. vol. 94. 1998;p. 695–8
  36. O'Conell J, Houston A, Bennett MW, O'Sullivan GC, Shanahan F. Immune privilege or inflammation? Insights into the FAS ligand enigma. Nat Med. 2001;7:271–274

PII: S0109-5641(05)00239-3

doi: 10.1016/j.dental.2005.05.013

Dental Materials
Volume 22, Issue 7 , Pages 630-640 , July 2006