Dental Materials
Volume 22, Issue 7 , Pages 622-629, July 2006

Fabrication and release behavior of a novel freeze-gelled chitosan/γ-PGA scaffold as a carrier for rhBMP-2

  • Chieh-Yang Hsieh

      Affiliations

    • Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, ROC
    • ,
  • Hsyue-Jen Hsieh

      Affiliations

    • Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, ROC
    • ,
  • Hwa-Chuang Liu

      Affiliations

    • Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan, ROC
    • ,
  • Da-Ming Wang

      Affiliations

    • Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan, ROC
    • ,
  • Lein-Tuan Hou

      Affiliations

    • Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University and Department of Periodontology, National Taiwan University Hospital, Taipei, Taiwan, ROC
    • Corresponding Author InformationCorresponding author. Tel.: +886 2 2312 3456x6854; fax: +886 2 2383 1346.

Received 8 February 2005; received in revised form 19 April 2005; accepted 16 May 2005.

Summary 

Objective

The aim of this study was to fabricate a novel composite porous scaffold by blending chitosan and gamma-poly(glutamic acid) (γ-PGA) for the sustained delivery of rhBMP-2.

Methods

Chitosan and γ-PGA were blended to fabricate a novel porous scaffold by the freeze-gelation method. For comparison, scaffolds made of freeze-dried chitosan, freeze-dried PLLA, and freeze-gelled chitosan were also prepared. The scaffolds were loaded with rhBMP-2, and then the controlled release of rhBMP-2 from the scaffolds was assessed by ELISA.

Results

The freeze-gelled chitosan/γ-PGA scaffold (M0=318.29ng, k=0.32d−1) gave the most satisfactory release curve, followed by the freeze-gelled chitosan (M0=392.76ng, k=0.59d−1), freeze-dried chitosan (M0=229.21ng, k=2.28d−1), and freeze-dried PLLA (M0=8.4ng, k=482.54d−1) scaffolds. In the stability test, p-dioxane (the solvent for PLLA) seriously deteriorated rhBMP-2, whereas acetic acid (the solvent for chitosan) did not.

Significance

A novel chitosan/γ-PGA composite scaffold for the controlled release of rhBMP-2 was established, with an enhanced release amount and sustained release behavior. This scaffold has many potential applications in bone regenerative therapies.

Keywords: Chitosan, γ-PGA, rhBMP-2, Controlled release

To access this article, please choose from the options below

Login to an existing account or Register a new account.

  • Purchase this article for 31.50 USD (You must login/register to purchase this article)

    Online access for 24 hours. The PDF version can be downloaded as your permanent record.

  • Subscribe to this title

    Get unlimited online access to this article and all other articles in this title 24/7 for one year.

  • Claim access now

    For current subscribers with Society Membership or Account Number.

  • Visit SciVerse ScienceDirect to see if you have access via your institution.
 

PII: S0109-5641(05)00238-1

doi:10.1016/j.dental.2005.05.012

Dental Materials
Volume 22, Issue 7 , Pages 622-629, July 2006

Article Tools

* Image Usage & Resolution