In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond

Imazato, Satoshi; Kuramoto, Akiko; Takahashi, Yusuke; Ebisu, Shigeyuki; Peters, Mathilde C.

doi:10.1016/j.dental.2005.05.009

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Volume 22, Issue 6 , Pages 527-532, June 2006

In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond

Satoshi Imazato
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
- Corresponding author. Tel.: +81 6 6879 2928; fax: +81 6 6879 2929.
Akiko Kuramoto
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Yusuke Takahashi
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Shigeyuki Ebisu
- Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan
Mathilde C. Peters
- Cariology, Restorative Sciences and Endodontics, School of Dentistry, The University of Michigan, 1011 N., D2361 Ann Arbor, MI, USA

Received 21 March 2005; accepted 11 May 2005.

Summary

Objectives

This study aimed to investigate the antibacterial effects of the dentin primer of a commercially available self-etching adhesive system, Clearfil Protect Bond, which contains antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB).

Methods

Inhibitory effects against Streptococcus mutans, Lactobacillus casei, or Actinomyces naeslundii were examined by an agar-disc diffusion method using the Clearfil Protect Bond primer containing 5% MDPB and an acidic adhesion-promoting monomer MDP, the primer only with MDP, and the primer with 1% cetylpyridinium chloride. The minimum inhibitory/bactericidal concentrations (MIC/MBC) of each primer for the three bacterial species were determined by serial microdilution assays. For testing the bactericidal effects seen in dentin, the primer was applied to demineralized dentin blocks in which S. mutans had been impregnated, and numbers of viable bacteria were counted.

Results

For all three bacteria, the sizes of the inhibition zones produced by Clearfil Protect Bond primer were significantly greater than for the other primers (p<0.05, ANOVA and Scheffe's F-test). The MIC/MBC values of Clearfil Protect Bond primer were less than those of the primer without MDPB, and comparable to those of the primer containing cetylpyridinium chloride. No bacterial recovery was obtained after application of Clearfil Protect Bond primer to the bacteria-impregnated dentin, although the primer without MDPB showed some bactericidal effect.

Significance

Clearfil Protect Bond primer has strong antibacterial activity based upon MDPB against S. mutans, L. casei and A. naeslundii, and the capability to disinfect cavities containing residual bacteria.

Keywords: Protect bond, Antibacterial activity, Adhesive system, MDPB, Self-etching primer, Cavity disinfecting effects