Dental Materials
Volume 22, Issue 6 , Pages 499-505, June 2006

Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts

  • J. Volk

      Affiliations

    • Department of Conservative Dentistry and Periodontology, Medical University Hannover, D-30625 Hannover, Germany
    • ,
  • J. Engelmann

      Affiliations

    • Department of Conservative Dentistry and Periodontology, Medical University Hannover, D-30625 Hannover, Germany
    • ,
  • G. Leyhausen

      Affiliations

    • Department of Conservative Dentistry and Periodontology, Medical University Hannover, D-30625 Hannover, Germany
    • ,
  • W. Geurtsen

      Affiliations

    • Department of Restorative Dentistry/Division of Operative Dentistry, School of Dentistry, University of Washington, P.O. Box 357456, Seattle, WA 98195 7456, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 206 543 5948; fax: +1 206 543 7783.

Received 21 March 2005; received in revised form 9 June 2005; accepted 16 June 2005.

Summary 

Objectives

Oral and systemic cells are permanently exposed to various types of xenobiotics, such as dental restorative materials, which may subsequently cause adverse effects. Objective of the present investigation was to analyze the effects of three important resin monomers on the glutathione metabolism of human gingival fibroblasts after an incubation period of 4h.

Methods

Cells were exposed to various concentrations of 2-hydroxyethyl methacrylate (HEMA; 0.1–10mM), triethylene-glycol dimethacrylate (TEGDMA; 0.05–2.5mM), and urethane dimethacrylate (UDMA; 0.005–0.25mM). Subsequently, cellular glutathione (GSH) concentrations were determined after a treatment period of 4h using the monobromobimane assay. Data were statistically evaluated using Tukey ANOVA with p<0.05.

Results

GSH depletion was dependent on the type of the resin monomer: UDMA>TEGDMA>HEMA. The concentrations for a 50%-reduction of cellular GSH varied between 0.1mM (0.05mM) (UDMA), 0.33mM (0.09mM) (TEGDMA), and 1.6mM (0.8mM) (HEMA). Simultaneously, no decrease of cell numbers was found at any tested concentration.

Significance

These data indicate that the investigated resins may cause cell damage due to depletion of intracellular GSH level even at low concentrations within a short period of time. The decrease of GSH is an early reaction, which is triggered prior to other cytotoxic alterations.

Keywords: Gingival fibroblasts, TEGDMA, HEMA, UDMA, Glutathione

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PII: S0109-5641(05)00222-8

doi:10.1016/j.dental.2005.06.002

Dental Materials
Volume 22, Issue 6 , Pages 499-505, June 2006

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