Influence of the application time on the penetration of different dental adhesives and a fissure sealant into artificial subsurface lesions in bovine enamel

Meyer-Lueckel, Hendrik; Paris, Sebastian; Mueller, Jan; Cölfen, Helmut; Kielbassa, Andrej M.

doi:10.1016/j.dental.2005.03.005

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Volume 22, Issue 1 , Pages 22-28, January 2006

Influence of the application time on the penetration of different dental adhesives and a fissure sealant into artificial subsurface lesions in bovine enamel

Hendrik Meyer-Lueckel
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Assmannshauserstr. 4-6, 14197 Berlin, Germany
Sebastian Paris
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Assmannshauserstr. 4-6, 14197 Berlin, Germany
- Corresponding author. Tel.: +49 30 8445 6207; fax: +49 30 8445 6204.
Jan Mueller
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Assmannshauserstr. 4-6, 14197 Berlin, Germany
Helmut Cölfen
- Department of Colloid Chemistry, Max-Planck-Institute of Colloids and Interfaces, Golm, Germany
Andrej M. Kielbassa
- Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité-Universitätsmedizin Berlin, Assmannshauserstr. 4-6, 14197 Berlin, Germany

Received 8 February 2005; accepted 14 March 2005.

Summary

Sealing of approximal enamel lesions by infiltration with low viscous resins seems to be a promising approach in non-operative dentistry and should bear advantages compared to remineralization or invasive treatment.

Objective

The aim of this in vitro study was to evaluate the penetration ability of five dental adhesives and a fissure sealant into initial enamel lesions for an application time of either 15s or 30s.

Methods

In each of 54 specimens of bovine enamel, three windows were demineralized for 14 days. Subsequently, two windows were etched with phosphoric acid for 5s in order to degrade the surface layer, whereas one window served as untreated control. The specimens were randomly divided into six groups and a fissure sealant as well as five different adhesives were applied onto the subsurface lesions and allowed to penetrate for either 15 or 30s. Overlying material was wiped away and the resins were light cured. To visualize the penetrated resins and the remaining pore structures, the specimens were infiltrated with a low viscous fluorescent resin and studied using a Confocal Laser Scanning Microscope (CLSM).

Results

For Helioseal, Heliobond, Resulcin Monobond, and Excite an application time of 30s resulted in significantly higher (p<0.05; t-test) penetration depths (47–105μm) compared to 15s (29–49μm).

Significance

Helioseal, Heliobond, Resulcin Monobond, and Excite are suitable for sealing artificial subsurface enamel lesions in vitro. After an application time of 30s a significant deeper penetration could be observed for these materials.

Keywords: Sealant, Approximal lesions, Bovine enamel, Adhesive, Penetration, VIRIN, CLSM